In this article, the authors describe a study into the factorial effect of selected process parameters on the pharmaceutical characteristics of polydllactidecoglycolide microspheres containing methotrexate. Sem was used to determine the size of the microspheres by measuring the diameter of 50 microspheres in an sem picture and then taking an average value. Preparation of uniformsized pla microcapsules by combining shirasu porous glass membrane. The aim of this paper was to further investigate the in vitro characteristics and in vivo performances of nalmefene microspheres. Process for preparation of microcapsules and microspheres 67.
Specific grade of polyethylene used in manufacturing of these microspheres is fdaapproved for food applications in chewing gum. Polymeric microspheres have gained widespread application as drug eluting depots. Moreover, plga microspheres can be injected directly into the action site, where the sirna can be released in controlled manner, thus avoiding the need of frequent invasive administrations. By default, the surfaces of plga microspheres are slightly negatively charged. Plga micronanoparticles that combine plga particles with the beneficial. Plga microspheres are widely studied for controlled release drug delivery applications, and many models have been proposed to describe plga degradation and erosion and drug release from the bulk. Polylactidecoglycolide microspheres plga ms are spherical, biodegradable polymer particles that can be loaded with a great variety of therapeutic molecules. Release of a woundhealing agent from plga microspheres in. Strategies for increasing drug loading in plgamicrospheres include modification of the classical solvent evaporation methods, preparation of multilayered microparticles, and development of novel methods for microparticle fabrication including hydrogel templates, coaxial electrospray, microfluidics, and scco 2. Plga microspheres loaded with kslw were prepared by using the doubleemulsification solvent evaporation method wow 1820, with plga 50. Polylacticcoglycolic acid plga is the most widely used biomaterial for. Preparation of proteinloaded microspheres using the woo h w method. Prepared using solvent evaporation methodibu could combine. For example, unpigmented or clear polyethylene microspheres supplied by cospheric in sizes from 10 micron to micron meet the quality requirements of the us fda as specified in 21 cfr 172.
We offer degradex plga microspheres and nanoparticles from 100 nm to 50. Protein encapsulation into plga nanoparticles by a novel phase separation method. Plain plga particles for testing system compatibility in drug delivery research. Composite plga microspheres containing exenatideencapsulated lecithin nanoparticles exnpsplgams were obtained by initial fabrication. The influences of inner aqueous phase, organic solvent, plga concentration on the morphology of microspheres were studied. In an in vitro study of drug release, it can be concluded that the bdmc plga ms exhibited sustained and longterm release properties for 96 h. To make our standard degradex plga nanospheres and microspheres, we use polyd,llactidecoglycolide with a lactic acid. First, 1 ml of drug or water was dispersed in 5 ml of different concentrations of plga, pla, and dichloromethane solutions by stirring at a rotation speed of 2500 rpm for one minute using a homogenizer fa25, fluko, shanghai, peoples republic of china. The size of the plga microspheres was also measured by a bi240 laser scattering particle sizer brookhaven.
Novel preparation method for sustainedrelease plga microspheres using waterinoilinhydrophilicoilinwater emulsion xiaoyun hong,1,2, liangming wei,3, liuqing ma,2 yinghui chen,4 zhenguo liu,1. Polymer and microsphere blending to alter the release of a. Microencapsulation of inorganic nanocrystals into plga. Bioerodable plgabased microparticles for producing. Plga microspheres were sieved by a 100mesh sieve before the millirod fabrication. The microspheres containing minocycline were prepared by the wow. Injectable and porous plga microspheres that form highly porous scaffolds at body temperature omar qutachia, jolanda r. Chapter 3 in combination with small sugars combine excellent stabilizing properties. Lipid and plga hybrid microparticles as carriers for. In this study, porous plga microspheres were fabricated by an emulsionsolvent.
Implantable hydrogel beads entrapping plgapaclitaxel. The purpose of this study was to develop and evaluate a novel composite microsphere delivery system composed of polyd,l. Solid biodegradable microspheres incorporating a drug. Aug 11, 2009 the aim of the present study was to investigate the morphology and function of a drug eluting metallic porous surface produced by the immobilization of poly lactidecoglycolide microspheres bearing dexamethasone onto plasma electrolytically oxidized ti6al7nb medical alloy. Pdf polyd,llacticcoglycolic acid plgapoly lactic acid pla. Plga particles are used for controlled release, sustained release and targeted drug delivery. Fabrication and characterization of controlled release. Prepared using solvent evaporation methodibu could combine with splla well and part of plla were degraded after releasing. Porous plga microspheres effectively loaded with bsa. Preparation and in vitroin vivo evaluation of insulin. Microspheres are manufactured in both solid and hollow form. The wet microspheres were then stirred at rpm for 2 hrs at 28c to permit evaporation of dcm and solidification of microspheres.
Magnetic microspheres18 this kind of delivery system is very much important which localizes the drug to the disease site. Biodegradation and biocompatibility of pla and plga. Plain degradex plga microspheres product data sheet. Nalmefene was blended with poly lactidecoglycolide. The influences of inner aqueous phase, organic solvent, plga concentration on. Pla microcapsules by combining shirasu porous glass membrane. They are approved by the us food and drug administration fda to be used as drug delivery system in humans. The aim of the present study was to investigate the morphology and function of a drug eluting metallic porous surface produced by the immobilization of poly lactidecoglycolide. A fundamental understanding of the in vivo biodegradation phenomenon as well as an appreciation of cellular and tissue responses which determine the biocompatibility of biodegradable pla and plga. Polylacticcoglycolic acid plga particles often serve as a biodegradable and biocompatible platform for drug delivery 1. It was shown that the microspheres from double emulsion. Development of composite plga microspheres containing.
Nov 25, 2014 polyd,llactidecoglycolide plga microspheres were prepared by emulsion solvent evaporation method. The results showed that addition of porogen or surfactants to the inner aqueous phase, types of organic solvents and polymer concentration affected greatly the. Recent research and development of plgapla microspheres. Typically, drugloaded polymeric microspheres are prepared by oilinwater emulsification which yields a product with. Poor patient compliance, untoward reactions and unstable blood drug levels after the bolus administration are impeding the pharmacotherapy for insobriety.
The release profile from the microspheres depends on the nature of the polymer used in the preparation as well as on the nature of the active drug. The present disclosure provides, inter alia, formulation compositions comprising modified nucleic acid molecules which may encode a protein, a protein precursor, or a partially or fully processed form of the. Plga microspheres are widely studied for controlled release drug delivery applications, and many models have been proposed to describe plga degradation and erosion and drug release from the bulk polymer. Preparation of plga microspheres with different porous. A longacting preparation may address these limitations.
Preparation of polylactidecoglycolide microspheres and. This work focuses on the development of poly lacticcoglycolic acid plga microspherepolyvinyl alcohol pva hydrogel composite coatings to permit longterm glucose sensor. Polyurethene, ureaformaldehyde, pmma, polystyrene 10 15 20 ncy % 0. The loading efficiency, the encapsulation efficiency, and the release profile of the bsaloaded plga microspheres were measured and studied. Autocatalysis is known to have a complex role in the dynamics of plga erosion and drug transport and can lead. Fabrication of alginate beads entrapping plgapaclitaxel microspheres by electrospray. The present disclosure provides, inter alia, formulation compositions comprising modified nucleic acid molecules which may encode a protein, a protein precursor, or a partially or fully processed form of the protein or a protein precursor. During some research on plga microspheres we found this interesting article published in european cells and materials vol 7 suppl 2. Polyd,llactidecoglycolide plga microspheres were prepared by emulsion solvent evaporation method. Us6855331b2 us10165,975 us16597502a us6855331b2 us 6855331 b2 us6855331 b2 us 6855331b2 us 16597502 a us16597502 a us 16597502a us 6855331 b2 us6855331 b2 us 6855331b2 authority us united states prior art keywords taxol release paclitaxel hydrophobic microspheres prior art date 19940516 legal status the legal status is an assumption and is not a legal conclusion. Poly lacticcoglycolic acid controlled release systems ncbi nih. Microspheres are made from polymeric, waxy or protective materials that is biodegradable synthetic polymers and modified natural products. Typically, drugloaded polymeric microspheres are prepared by oilinwater emulsification which yields a product with a broad size distribution.
The microspheres containing the sbm7462 peptide were prepared using the biodegradable plga copolymer 50. Polyacryloyl hydroxyethyl starch plga composite microspheres ge jiang, 1wei qiu, and patrick p. However, monolithic or singlewalled plga microspheres and. The aim of the present study was to investigate the properties of different sizefractions of drugloaded microspheres, in order to delineate whether particle size. First, 1 ml of drug or water was dispersed in 5 ml of different concentrations of plga, pla, and dichloromethane solutions by stirring. Novel preparation method for sustainedrelease plga. Pharmaceutics free fulltext risperidoneloaded plga. Sem images of plga microspheres covered with ho8910 cells. Several publications describe alternative plga formulations for the controlled release of risperidone. University of groningen protein delivery from polymeric. Studies of plga microspheres pharmaceutical technology. Use of biodegradable plga microspheres as a slow release. Two sustained release formulations, one incorporating 20% brij 98 and the other incorporating 3% mgco 3 in the oil phase. The size of mp showed gaus distribution and 90% of the particles d0.
The aim of this study was to design and evaluate biodegradable plga microspheres for sustained delivery of risperidone, with an eventual goal of avoiding combination therapy for the treatment of. Three formulations having different organic to water ratio of the primary emulsion were prepared. The release of drug from both biodegradable as well as nonbiodegradable microspheres is influenced by structure or micromorphology of the carrier and the properties of the polymer itself. The aim of this study was to design and evaluate biodegradable plga microspheres for sustained delivery of risperidone, with an eventual goal of avoiding combination therapy for the treatment of schizophrenia. The plga particle formation takes place spontaneously at the nucleation spots that are distributed randomly. Novel preparation method for sustainedrelease plga microspheres using waterinoilinhydrophilicoilinwater emulsion xiaoyun hong,1,2, liangming wei,3, liuqing ma,2 yinghui chen,4 zhenguo liu,1 weien yuan2, 1department of neurology, xinhua hospital affiliated to shanghai jiaotong university, school of medicine, shanghai, peoples republic of china. Effect of particle size on drug loading and release. A study of the microspheres stability at refrigerated temperatures is also examined. In vitro and in vivo evaluations of plga microspheres. The release characteristics of different model drugs from. The formulation composition may further include a modified nucleic acid molecule and a delivery agent. The encapsulation efficiency and in vitro release profiles of different drugs with various water solubilities from medicated plga microspheres were investigated. Polylacticcoglycolic acid plga is the most widely used biomaterial for microencapsulation and prolonged delivery of therapeutic drugs, proteins and antigens. Pdf biodegradation and biocompatibility of pla and plga.
Us6855331b2 sustained release hydrophobic bioactive plga. Microspheres are manufactured in both solid and hollow. European journal of pharmaceutics and biopharmaceutics. Pdf plga microspheres containing minocycline as drug. It was shown that the microspheres from double emulsion had smaller particle sizes 350 m, a less porous structure, a poor loading efficiency 5. Sem was used to determine the size of the microspheres by measuring the diameter of 50 microspheres in an sem. Jun 28, 2016 polylacticcoglycolic acid plga is the most widely used biomaterial for microencapsulation and prolonged delivery of therapeutic drugs, proteins and antigens. Among different forms of plgabased drug delivery systems, microspheres or microparticles are the most common. Among different forms of plga based drug delivery systems, microspheres or microparticles are the most common.
Hollow microspheres are used as additives to lower the density of a material. The dolomite centre ltd continuous microfluidic synthesis. Gastroretentive floating microspheres are lowdensity systems that have. Particles can be used as carriers by attaching drug or biologicalchemical entities to particle surface. Physicochemical characterization and pharmacokinetics of agomelatineloaded plga microspheres for intramuscular injection. However, commonly, the presence of lag times, sigmoidal release curves, and rather short release. Plga microspheres flexion therapeutics for musculoskeletal conditions. The wet microspheres obtained were collected by centrifugation followed by filtration and lyophillization 2,3,4,5.
Design of controlled release plga microspheres for. Plga containing solvent acetone and the antisolvent water form an azeotropic mixture. Injectable and porous plga microspheres that form highly. We offer a full range of degradex plga microspheres and plga nanoparticles that are ideal for a variety of applications poly d,llactidecoglycolide or plga microspheres and nanoparticles are known to possess a unique ability to enable drug release in a controlled manner. A fundamental understanding of the in vivo biodegradation phenomenon as well as an appreciation of cellular and tissue responses which determine the biocompatibility of biodegradable pla and plga microspheres are important components in the design and development of biodegradable microspheres containing bioactive agents for therapeutic application.
Solid biodegradable microspheres incorporating a drug dispersed or dissolved throughout particle matrix have the potential for controlled release of the drug. The particle size of different lipid and plga hybrid mp was between 10 and 25. Mar 22, 2012 spllaibuprofin microspheres 2010these are star shaped polyl lactideloaded ibuprofen spllaibu microspheres. The drug encapsulating efficiency of spllaibumicrospheres is high and release of ibuprofen. The effect of addition of mgco 3, drug loading, and polymer blending on the release of fenretinide from plga microspheres was also investigated and observed to enhance the drug release. Bharadia, vikram pandya and darshan modi department of pharmaceutics, b. Pdf preparation of openclosed pores of plgamicrosphere for.
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